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Conformational
Changes in Human DNA Characterize the Radiated
Energy from the Aulterra Formulation
Glen Rein, Ph.D.
Quantum Biology Research Labs
PO Box 157,
Northport, NY 11768
Introduction
Information content associated with both classical and non-classical
(subtle, non-Hertzian, scalar) electromagnetic energy can be stored
in physical objects. These objects include water (Rein, 1992, Schwartz,
1991), geometric patterns (Rein, 1997), electronic circuits (Dibble
& Tiller, 1999) and even paper (Omura, 1990). For water and electronic
circuits, preliminary evidence suggests that the stored information
can be subsequently retrieved or utilized by biological systems, thereby
producing a biological effect. In this sense these objects have a memory
of the information stored within them. Although long-term storage of
information in physical objects is considered an anomaly in the eyes
of traditional science, it has been demonstrated experimentally.
The author's own research in this area has demonstrated that subtle
energy generated from "free energy" devices and those associated with
human intention can be stored in water for several months if the optical
properties of water are measured using a special form of ultraviolet
(UV) spectroscopy (Rein, 1992). Furthermore, the stored energy is biologically
active when the water is exposed to a variety of biological systems
(Gagnon and Rein, 1990). One of the biological targets used in these
studies was the DNA molecule. In addition to these responses to stored
or imprinted energy, DNA has been shown to act as an antenna for other
forms of subtle energy (Rein, 1994a, 1996, 1997). Thus, it has been
discovered that in addition to classical EM fields (Semin, 1995), subtle
energy resonates with the DNA molecule and causes physical changes in
its secondary structure (winding and unwinding of the helix).
Goals
of this Research Project
1. Obtain scientific evidence that the energetic information imprinted
in the Aulterra powder can produce measurable and reproducible biological
effects on DNA using state-of-the art scientific methodology.
2. Measure the ability of Aulterra's powder to neutralize the harmful
effect from heavy metal toxicity.
3. Demonstrate a biological effect of the energetic information imprinted
in Aulterra's homeopathic preparation.
The
Experimental Approach:
In order to measure biological effects of subtle energy, a relatively
high degree of coupling or resonance between the energy and the matter
(bio-molecules) is required. The specific experimental conditions required
for such resonance depends on the nature of the subtle energy involved,
as well as the properties of the bio-molecule and its aqueous environment.
It is predicted that biological systems expressing quantum properties
(macroscopic quantum coherence) are most likely to resonate with subtle
energy. In addition to its known sensitivity to subtle energy, DNA was
chosen for these experiments since it exhibits quantum coherence (Popponin,
1998).
The experimental methodology used in this study involves measuring
the recovery of DNA after heat shock. Heat and other noxious stimuli
like heavy metals are know to break the hydrogen bonds, which hold the
two strands of the DNA helix together. Thus these harmful agents cause
the two strands to unwind. In the case of heat shock it is well known
that as the DNA cools it recovers and spontaneously rewinds back into
its original helical conformation (Thomas, 1954). The rewinding is a
natural recovery process that involves reforming hydrogen bonds. Since
the quantum properties of the hydrogen bond have been previously established,
it can be concluded that this assay is measuring the quantum properties
of DNA.
The recovery process can be monitored using an absorption spectrophotometer,
which can measure the amount of rewinding as a function of time. This
is a standard method in the biochemistry literature for measuring the
secondary structure of DNA (Thomas, 1954).
The rewinding slope is negative (downward) because as the DNA rewinds
it absorbs less light. The larger the negative number of the slope,
the faster is the recovery.
The
Specific Protocol
The exact protocol that was followed is confidential, but generally
speaking it involved heating a diluted solution of human placental DNA
(Sigma Chemical Co., St. Louis). Immediately after heat treatment, the
DNA was gently transferred to a quartz cuvette and then placed in the
cuvette holder inside the spectrophotometer. For all experiments, after
heat shock, the conformation of DNA was automatically measured every
60 seconds over the course of one hour, as the absorption of UV light
at 260nm. Absorption of light was measured using a UV-visible diode
array spectrophotometer (Hewlett Packard 8451A). In order to quantify
the speed of recovery, the slopes of the rewinding curves were calculated
using the IBM Excel software. For the data presented in Figures 1-3
only a portion of the entire recovery curve was analyzed. The light
gray line in these figures is the raw absorption data collected by the
spectrophotometer, whereas the solid black line is the computer generated
best-fit calculation of the slope (r2). For experiments with heavy metals,
the slope was calculated for each separate experiment and then compared
statistically using a two sample t-test (assuming equal variance).
DNA recovery to heat shock was measured:
1.) After a test tube of DNA sat on the Aulterra powder for varying
amounts of time
2.) After the DNA was exposed to varying amounts of the heavy metal,
copper.
3.) After the DNA solution containing copper was placed on the Aulterra
powder for varying amounts of time.
4.) After adding the Aulterra homeopathic preparation to the DNA (1/10
dilution).
Results and Discussion:
1. DNA exposed to the energy of the Aulterra powder
The recovery curve in Figure 1A is a typical curve when DNA is suspended
in water and left to spontaneously rewind. When the DNA was placed on
the Aulterra powder for one day and then measured, there was no change
in the recovery curve. However, after 3, 4 or 5 days of exposure
to the energy from the powder, a rather interesting recovery curve is
obtained (Figure 1B). Instead of a smooth and gradual rewinding process,
the recovery curve is oscillatory in nature. This means that the DNA
rewinds, then unwinds a little, then continues to rewind and continues
to go through cycles of winding and unwinding. This oscillatory behavior
is a characteristic of quantum coherence which has been observed in
other systems (Hideshima, 1990; Dibble & Tiller,1999). Thus,
it can be concluded that the energy from Aulterra induces quantum behavior
in DNA which is expressed as oscillatory behavior.
It is interesting to note that in the case of Tiller's experiments,
oscillatory behavior of water was induced by human intention associated
with healing states of consciousness. Healing states of consciousness
are known to be associated with coherent oscillatory EEG patterns (Schwartz
and Russek, 1997). Furthermore, it was demonstrated that the energy
from healers could effect the conformation of DNA only when the electrical
activity of their ECG exhibited such oscillatory behavior (Rein &
McCraty, 1994b). Thus, by inference the energy emitted from the Aulterra
powder may be similar to the energies associated with healers.
Neutralizing the effects of heavy metal
toxicity
Copper (Cu(II)) was chosen as a representative heavy metal because
the biological action of copper is mediated by binding to DNA and causing
it to unwind (Sagripanti, 1991). A series of experiments were done to
determine the appropriate concentration of copper, i.e. a high enough
concentration that would produce a measurable effect on DNA but not
too high to totally overload the system. It was predicted that the ability
of the energy from Aulterra's powder to neutralize the toxic effect
of copper would be minimized at higher concentrations of copper. Based
on the literature a concentration of 1mM was initially tested. In order
to determine the effect of copper the slope of the recovery curves needed
to be analyzed first in the absence of any heavy metals. The normal
recovery rate was determined in six separate experiments with the average
value for the slope of -0.813 ± 0.06. The recovery rate was then measured
in four separate experiments when DNA was exposed to 1mM copper for
2 days. The average slope was dramatically reduced to -0.023 ±
0.015. These results are presented in Figure 2 for two typical experiments
with and without copper. Preliminary experiments were conducted to determine
whether this large effect of copper on DNA recovery rates could be neutralized
if the DNA was placed on top of the Aulterra powder while being exposed
to the copper. After two days the average slope was -0.011, which is
not much different than -0.023 (both giving around 98% inhibition) indicating
that the energy from the Aulterra powder did not neutralize the damaging
effect of the copper. However, after three days the slope was -0.28
(66% inhibition) and after four days the slope was -0.36 (55% inhibition),
rapidly approaching the -0.81 value for the slope in the absence of
copper. These results demonstrate that the energy from the Aulterra
powder does in fact partially neutralize the toxic effect of copper
by reducing the 97% inhibition (with no energy) to 55% in the presence
of energy. The effect appears to be linear with time indicating that
the longer the DNA was exposed to the energy, the larger its neutralizing
ability. The results were encouraging but only revealed a partial protection
from copper when used at 1mM concentrations.
It is possible that only partial protection occurred because the concentration
of the toxin was too high. Therefore, these experiments were repeated
with a lower dose of copper, 0.5mM. Based on the previous experiments,
it was predicted that a neutralizing effect of the low concentration
of copper would be seen after only two days. Therefore, twelve separate
experiments were done with DNA exposed to 0.5mM copper in the presence
and absence of energy from Aulterra's powder (Table 1). In this case
the copper alone caused an 86% inhibition of DNA recovery, giving an
average slope value of -0.11 ± 0.25. In the presence of
the energy from the Aulterra powder, this effect was reduced to only
36%, giving an average slope of -0.52 ± 0.18. Typical experiments
for each of these conditions are shown in Figure 3. The raw data from
each of the 12 separate experiments is shown in Table 1. Statistical
comparison of the last two numbers reveals a highly significant difference
(p=0.01). These results, taken together with the previous results using
higher concentrations of copper (Table 2), clearly demonstrate the ability
of the energy from Aulterra's powder to neutralize the toxic effect
of copper.
|
Table 1
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Experiment
|
Off Power
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Experiment
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On Power
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|
1
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-0.219
|
7
|
-0.566
|
|
2
|
-0.499
|
8
|
-0.401
|
|
3
|
-0.02
|
9
|
-0.224
|
|
4
|
+0.127
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10
|
-0.525
|
|
5
|
-0.206
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11
|
-0.710
|
|
6
|
+0.166
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12
|
-0.662
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|
Average
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-0.109
|
Average
|
-0.516
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Standard Deviation
|
0.25
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Standard Deviation
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0.18
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Raw data from six experiments showing R2 slope values
for slope calculated by computer
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Table 2
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.
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Average Slope
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% Inhibition
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Standard
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No cu, no energy (control)
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-0.813
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0.06
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1.0mM Cu, no energy
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-0.023
|
97
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0.015
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10mM Cu with Aulterra's Energy
|
-0011
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98
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**
|
|
0.5mM Cu, no energy
|
-0.11
|
86
|
0.25
|
|
0.5mM with Aulterra's energy
|
-.052
|
36
|
0.18
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3.
The effect of Aulterra's homeopathic preparation on DNA.
Control water used to make the homeopathic preparation showed similar
behavior to that previously observed with deionized water. Preliminary
experiments indicated that, depending on the experiment, several different
types of responses were observed in DNA exposed to the homeopathic preparation.
Sometimes the recovery would initially appear normal (a steady decrease)
but after approximately 20 minutes the DNA would stop rewinding and
begin the slowly unwind (positive slope). In other cases it would stop
rewinding and remain stationary, i.e. didn't continue to wind or unwind
(flat slope). In other cases oscillatory behavior was observed very
similar to that observed when the DNA was placed on the powder (see
Figure 1B). This oscillatory behavior may be a characteristic response
of DNA to Aulterra's energy, whether it be radiating from a powder or
"read" by the DNA from information in imprinted water. The non-reproducibility
of the phenomenon in regards to the homeopathic preparation is likely
to be due to the fact that the resonant conditions have not yet been
found experimentally. It is predicted that under the correct resonance
conditions, the oscillatory behavior will be more reproducible.
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